polyclonal antibodies to rac1 and control peptides for preabsorption of the antibody Search Results


rac1  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology rac1
Figure 3. V12cdc42 and V14rhoA induce filopodia and stress fibers, respectively, in B16F1 cells. B16F1 cells transiently transfected with V12cdc42 (a, b) and V14rhoA (c, d) or A35rac1 (e, f) were detected by staining with antibodies to the c-myc epitope (a, c, e), and their cytoskeleton was visualized by the addition of rhodamine phalloidin with the secondary antibody (b, d, f). Cdc42-expressing cells exhibited fine filopodia (a, b), whereas V14rhoA expressing cells (c) were flattened and contained numerous dense and closely packed actin stress fibers (d). Cells expressing the inactive <t>rac1</t> protein A35rac1 resembled nontransfected cells, and displayed a polygonal morphology (e) with a sparse actin cytoskeleton (f). Scale bars: 5 µm.
Rac1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibodies+to+rac1+and+control+peptides+for+preabsorption+of+the+antibody/Rac+1/pm09699725-48-14-26
Average 95 stars, based on 1 article reviews
rac1 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier
c-myc antibody  (NSJ Bioreagents)
99
NSJ Bioreagents c-myc antibody
Figure 3. V12cdc42 and V14rhoA induce filopodia and stress fibers, respectively, in B16F1 cells. B16F1 cells transiently transfected with V12cdc42 (a, b) and V14rhoA (c, d) or A35rac1 (e, f) were detected by staining with antibodies to the c-myc epitope (a, c, e), and their cytoskeleton was visualized by the addition of rhodamine phalloidin with the secondary antibody (b, d, f). Cdc42-expressing cells exhibited fine filopodia (a, b), whereas V14rhoA expressing cells (c) were flattened and contained numerous dense and closely packed actin stress fibers (d). Cells expressing the inactive <t>rac1</t> protein A35rac1 resembled nontransfected cells, and displayed a polygonal morphology (e) with a sparse actin cytoskeleton (f). Scale bars: 5 µm.
C Myc Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibodies+to+rac1+and+control+peptides+for+preabsorption+of+the+antibody/c-Myc+Antibody/custom%40f52056%409699725
Average 99 stars, based on 1 article reviews
c-myc antibody - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

Image Search Results


Figure 3. V12cdc42 and V14rhoA induce filopodia and stress fibers, respectively, in B16F1 cells. B16F1 cells transiently transfected with V12cdc42 (a, b) and V14rhoA (c, d) or A35rac1 (e, f) were detected by staining with antibodies to the c-myc epitope (a, c, e), and their cytoskeleton was visualized by the addition of rhodamine phalloidin with the secondary antibody (b, d, f). Cdc42-expressing cells exhibited fine filopodia (a, b), whereas V14rhoA expressing cells (c) were flattened and contained numerous dense and closely packed actin stress fibers (d). Cells expressing the inactive rac1 protein A35rac1 resembled nontransfected cells, and displayed a polygonal morphology (e) with a sparse actin cytoskeleton (f). Scale bars: 5 µm.

Journal: The Journal of investigative dermatology

Article Title: Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model.

doi: 10.1046/j.1523-1747.1998.00276.x

Figure Lengend Snippet: Figure 3. V12cdc42 and V14rhoA induce filopodia and stress fibers, respectively, in B16F1 cells. B16F1 cells transiently transfected with V12cdc42 (a, b) and V14rhoA (c, d) or A35rac1 (e, f) were detected by staining with antibodies to the c-myc epitope (a, c, e), and their cytoskeleton was visualized by the addition of rhodamine phalloidin with the secondary antibody (b, d, f). Cdc42-expressing cells exhibited fine filopodia (a, b), whereas V14rhoA expressing cells (c) were flattened and contained numerous dense and closely packed actin stress fibers (d). Cells expressing the inactive rac1 protein A35rac1 resembled nontransfected cells, and displayed a polygonal morphology (e) with a sparse actin cytoskeleton (f). Scale bars: 5 µm.

Article Snippet: Monoclonal antibodies to the c-myc epitope (9E10) were purchased from Sigma; polyclonal antibodies to rac1 and control peptides for preabsorption of the antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Transfection, Staining, Expressing

Figure 6. A dominant negative inhibitor of rac1 (N17rac1) abrogates the ability of NDP-MSH to induce dendrites in B16F1 cells. B16F1 cells were transiently transfected with N17rac1 (a–d), N17cdc42 (e), or A35rac1 (f) overnight and cells were then treated with NDP-MSH (10–8 M) for 24 h. Transfected cells were detected by staining with antibodies to the c-myc epitope (a, c, e, f); the actin cytoskeleton was detected by staining with rhodamine phalloidin (b, d). Cells expressing N17rac1 (a, c; c-myc epitope) were round or polygonal and did not contain dendrites. The actin cytoskeleton was sparse (b, d), similar to nontransfected cells. In contrast, cells expressing N17cdc42 or A35rac1 (e and f, respectively; c-myc epitope) formed branching dendritic structures in response to NDP-MSH. Scale bars: 10 µm.

Journal: The Journal of investigative dermatology

Article Title: Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model.

doi: 10.1046/j.1523-1747.1998.00276.x

Figure Lengend Snippet: Figure 6. A dominant negative inhibitor of rac1 (N17rac1) abrogates the ability of NDP-MSH to induce dendrites in B16F1 cells. B16F1 cells were transiently transfected with N17rac1 (a–d), N17cdc42 (e), or A35rac1 (f) overnight and cells were then treated with NDP-MSH (10–8 M) for 24 h. Transfected cells were detected by staining with antibodies to the c-myc epitope (a, c, e, f); the actin cytoskeleton was detected by staining with rhodamine phalloidin (b, d). Cells expressing N17rac1 (a, c; c-myc epitope) were round or polygonal and did not contain dendrites. The actin cytoskeleton was sparse (b, d), similar to nontransfected cells. In contrast, cells expressing N17cdc42 or A35rac1 (e and f, respectively; c-myc epitope) formed branching dendritic structures in response to NDP-MSH. Scale bars: 10 µm.

Article Snippet: Monoclonal antibodies to the c-myc epitope (9E10) were purchased from Sigma; polyclonal antibodies to rac1 and control peptides for preabsorption of the antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Dominant Negative Mutation, Transfection, Staining, Expressing

Figure 7. A dominant negative inhibitor of rac1 abrogates the ability of UV light to induce dendrites in B16F1 cells. B16F1 cells were transiently transfected with N17cdc42 (a), A35rac1 (b), or N17rac1 (c, d) overnight and cells were then irradiated with a single dose of UV light from a solar simulator (4.0 J per cm2). Twenty-four hours later transfected cells were detected by immunofluorescence staining for the c-myc epitope tag (a–d). Cells expressing N17cdc42 (a) and A35rac1 (b) responded to UV light with the formation of dendrites; in contrast, cells expressing N17rac1 (c, d) failed to form dendrites in response to UV light. The actin cytoskeleton of these cells was sparse and did not contain stress fibers (not shown). Scale bars: 15 µm.

Journal: The Journal of investigative dermatology

Article Title: Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model.

doi: 10.1046/j.1523-1747.1998.00276.x

Figure Lengend Snippet: Figure 7. A dominant negative inhibitor of rac1 abrogates the ability of UV light to induce dendrites in B16F1 cells. B16F1 cells were transiently transfected with N17cdc42 (a), A35rac1 (b), or N17rac1 (c, d) overnight and cells were then irradiated with a single dose of UV light from a solar simulator (4.0 J per cm2). Twenty-four hours later transfected cells were detected by immunofluorescence staining for the c-myc epitope tag (a–d). Cells expressing N17cdc42 (a) and A35rac1 (b) responded to UV light with the formation of dendrites; in contrast, cells expressing N17rac1 (c, d) failed to form dendrites in response to UV light. The actin cytoskeleton of these cells was sparse and did not contain stress fibers (not shown). Scale bars: 15 µm.

Article Snippet: Monoclonal antibodies to the c-myc epitope (9E10) were purchased from Sigma; polyclonal antibodies to rac1 and control peptides for preabsorption of the antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Dominant Negative Mutation, Transfection, Irradiation, Staining, Expressing

Figure 8. NDP-MSH and UV light induce the localization of rac1 to dendritic tips. B16F1 cells were either untreated (a), were treated with NDP- MSH (b) for 24 h (10–8 M), or were irradiated with a single dose of UV light from a solar simulator (c; 4.0 J per cm2) and rac1 localization was detected by immunofluorescence staining and slides examined by confocal microscopy. Negative controls consisted of cells stained with rac1 antibodies preabsorbed with rac1 peptide (d). Rac1 is primarily located in the cell cytoplasm in untreated cells (a). NDP-MSH treated and UV irradiated cells exhibit dendritic cell processes in which rac1 is localized to dendrite tips (arrowheads; b, c). Scale bar : 7 µm.

Journal: The Journal of investigative dermatology

Article Title: Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model.

doi: 10.1046/j.1523-1747.1998.00276.x

Figure Lengend Snippet: Figure 8. NDP-MSH and UV light induce the localization of rac1 to dendritic tips. B16F1 cells were either untreated (a), were treated with NDP- MSH (b) for 24 h (10–8 M), or were irradiated with a single dose of UV light from a solar simulator (c; 4.0 J per cm2) and rac1 localization was detected by immunofluorescence staining and slides examined by confocal microscopy. Negative controls consisted of cells stained with rac1 antibodies preabsorbed with rac1 peptide (d). Rac1 is primarily located in the cell cytoplasm in untreated cells (a). NDP-MSH treated and UV irradiated cells exhibit dendritic cell processes in which rac1 is localized to dendrite tips (arrowheads; b, c). Scale bar : 7 µm.

Article Snippet: Monoclonal antibodies to the c-myc epitope (9E10) were purchased from Sigma; polyclonal antibodies to rac1 and control peptides for preabsorption of the antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Irradiation, Staining, Confocal Microscopy